Journal: Cancer Management and Research

Article Title: Regulatory Role and Mechanism of lncRNA RNF217-AS1 in the Proliferation and Migration of Esophageal Cancer Cells

doi: 10.2147/cmar.s515036

Figure Lengend Snippet: Figure 1 Comparison of RNF217-AS1 expression across various esophageal cancer cell lines. Note: Compared to HET1A cells, **P<0.01, ***P<0.0001.

Article Snippet: Experimental Cells and Main Reagents Esophageal cancer cell lines (EC9706, Eca109, KYSE-510, TE-13) and the immortalized esophageal epithelial cell line (HET-1A) were procured from the American Type Culture Collection (ATCC).

Techniques: Comparison, Expressing

Journal: Cancer Management and Research

Article Title: Regulatory Role and Mechanism of lncRNA RNF217-AS1 in the Proliferation and Migration of Esophageal Cancer Cells

doi: 10.2147/cmar.s515036

Figure Lengend Snippet: Figure 2 Effect of RNF217-AS1 knockdown on the proliferation activity of esophageal cancer l cells. (A) The knockdown of RNF217-AS1 expression by siRNA was verified by RT-qPCR in Eca109 and KYSE510 cells; (B) Colony formation assay demonstrated that knockdown of RNF217-AS1 inhibited proliferation activity of esophageal cancer cells (crystal violet staining). Note: Compared to si-NC group, **P<0.01, ***P<0.001.

Article Snippet: Experimental Cells and Main Reagents Esophageal cancer cell lines (EC9706, Eca109, KYSE-510, TE-13) and the immortalized esophageal epithelial cell line (HET-1A) were procured from the American Type Culture Collection (ATCC).

Techniques: Knockdown, Activity Assay, Expressing, Quantitative RT-PCR, Colony Assay, Staining

Journal: Cancer Management and Research

Article Title: Regulatory Role and Mechanism of lncRNA RNF217-AS1 in the Proliferation and Migration of Esophageal Cancer Cells

doi: 10.2147/cmar.s515036

Figure Lengend Snippet: Figure 3 Effect of RNF217-AS1 knockdown on the migration of esophageal cancer cells (×200 magnification for KYSE510 cells, ×100 magnification for Eca109 cells). Note: Compared to si-NC group, **P<0.01.

Article Snippet: Experimental Cells and Main Reagents Esophageal cancer cell lines (EC9706, Eca109, KYSE-510, TE-13) and the immortalized esophageal epithelial cell line (HET-1A) were procured from the American Type Culture Collection (ATCC).

Techniques: Knockdown, Migration

Journal: Cancer Management and Research

Article Title: Regulatory Role and Mechanism of lncRNA RNF217-AS1 in the Proliferation and Migration of Esophageal Cancer Cells

doi: 10.2147/cmar.s515036

Figure Lengend Snippet: Figure 4 RNF217-AS1 targeted and negatively regulated the expression of miR-377-3p in esophageal cancer cells. (A) LncRNA2 Target analysis of the binding site between RNF217-AS1 and miR-377-3p; (B) Effect of RNF217-AS1 knockdown on miR-377-3p expression (Compared to si-NC group, **P<0.01, ***P<0.001); (C) Dual-luciferase reporter assay validating the targeting relationship between RNF217-AS1 and miR-377-3p (Compared to RNF217-AS1-WT, **P<0.01).

Article Snippet: Experimental Cells and Main Reagents Esophageal cancer cell lines (EC9706, Eca109, KYSE-510, TE-13) and the immortalized esophageal epithelial cell line (HET-1A) were procured from the American Type Culture Collection (ATCC).

Techniques: Expressing, Binding Assay, Knockdown, Luciferase, Reporter Assay

Journal: Cancer Management and Research

Article Title: Regulatory Role and Mechanism of lncRNA RNF217-AS1 in the Proliferation and Migration of Esophageal Cancer Cells

doi: 10.2147/cmar.s515036

Figure Lengend Snippet: Figure 5 Effects of RNF217-AS1 knockdown on the expression of epithelial-mesenchymal transition (EMT) related proteins in esophageal cancer cells (Western Blots). Note: Compared to si-NC, **P<0.01, ***P<0.001.

Article Snippet: Experimental Cells and Main Reagents Esophageal cancer cell lines (EC9706, Eca109, KYSE-510, TE-13) and the immortalized esophageal epithelial cell line (HET-1A) were procured from the American Type Culture Collection (ATCC).

Techniques: Knockdown, Expressing, Western Blot

Journal: Cell Communication and Signaling : CCS

Article Title: In vitro effect of hCG on cryptorchid patients’ gubernacular cells: a predictive model for adjuvant personalized therapy

doi: 10.1186/s12964-024-01979-y

Figure Lengend Snippet: Gubernacular cells derived from cryptorchid patients grow in vitro. A Representative bright field images of gubernacular cells derived from cryptorchid patients #2, #3, #7, #20, and #94, and of cells derived from control patients #15, #40, #55, and #116. Scale bar: 100 μm. B Proliferation rate of gubernacular cells derived from patients #2, #7, #20, and #94 (black symbols), and of cells derived from control patients #40 and #116 (white symbols). Values are reported as fold change relative to the beginning of the experiment (T0) and are the means (± SEM) of four independent biological replicates. Statistical legend: p < 0.5 (*) for pt #94 versus pt #7, #20, #40, and #116 after 7 days; p < 0.5 (*) for pt #94 versus pt #7, #20, #2, and #116 after 10 days. Based on these curves, we also report patients’ doubling time (days) and the significance relative to pt #94 derived cells

Article Snippet: B Heat-map representation of the number of junctions and the main of branching length (MBL) of hCG-treated gubernacular cells derived from cryptorchid patients (#94, #2, #7, #20, and #3) and control patients (#116, #40, and #15) at four different time points: after 3 (T1), 6 (T2), 9 (T3), and 12 (T4) hours from the seeding.

Techniques: Derivative Assay, In Vitro, Control

Journal: Cell Communication and Signaling : CCS

Article Title: In vitro effect of hCG on cryptorchid patients’ gubernacular cells: a predictive model for adjuvant personalized therapy

doi: 10.1186/s12964-024-01979-y

Figure Lengend Snippet: Analysis of gonadotropin and androgen receptor expression in gubernacular cells cultured in vitro. A mRNA expression levels of LHCGR full-length, LHCGR short-length, FSHR , and AR in five cryptorchid patients’ derived cells (pt #2, #3, #7, #20, and #94) and three control patients (pt #15, #40, and #116). The mRNA levels of the receptors have been normalized on the housekeeping gene succinate dehydrogenase ( SDHA ) expression and reported as fold change relative to the levels of expression in testicular tissue derived from the control adult patient #40. Values are the means (± SEM) of four independent biological replicates. Statistical legend: (*) p < 0.05 versus pt #40. B Representative immunofluorescent LHCGR expression (green) images in gubernacular cells derived from cryptorchid patients (#2, #3, #7, #20, and #94), and from controls (#15, #40, and #116). Nuclei are marked with DAPI in blue and LHCGR long-variant in green. Scale bar: 50 μm. C RXFP2 expression values reported as the difference (D CT) with the SDHA housekeeping gene for each single sample. Values are the means (± SEM) of four independent biological replicates. n.d .: not determined, meaning that this sample does not express at any level the RXFP2 gene

Article Snippet: B Heat-map representation of the number of junctions and the main of branching length (MBL) of hCG-treated gubernacular cells derived from cryptorchid patients (#94, #2, #7, #20, and #3) and control patients (#116, #40, and #15) at four different time points: after 3 (T1), 6 (T2), 9 (T3), and 12 (T4) hours from the seeding.

Techniques: Expressing, Cell Culture, In Vitro, Derivative Assay, Control, Variant Assay

Journal: Cell Communication and Signaling : CCS

Article Title: In vitro effect of hCG on cryptorchid patients’ gubernacular cells: a predictive model for adjuvant personalized therapy

doi: 10.1186/s12964-024-01979-y

Figure Lengend Snippet: Effect of hCG on gubernacular cell proliferation. The effect of 100 IU/mL hCG on cryptorchid and control patients’ cell proliferation has been evaluated by analyzing the data of hCG-treated cells (corresponding to the histograms) with those of untreated cells (dashed line), whose proliferation corresponds to 100%, for the same patient. White histograms represent control patients (#116, #40, #15, and #55), whereas grey histograms represent cryptorchid patients (#94, #2, #7, #20, and #3). Values are the means (± SEM) of four independent biological replicates. Statistical legend: p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) for treated cells versus untreated cells of the same patient or, when indicated, among hCG-treated patient cells

Article Snippet: B Heat-map representation of the number of junctions and the main of branching length (MBL) of hCG-treated gubernacular cells derived from cryptorchid patients (#94, #2, #7, #20, and #3) and control patients (#116, #40, and #15) at four different time points: after 3 (T1), 6 (T2), 9 (T3), and 12 (T4) hours from the seeding.

Techniques: Control

Journal: Cell Communication and Signaling : CCS

Article Title: In vitro effect of hCG on cryptorchid patients’ gubernacular cells: a predictive model for adjuvant personalized therapy

doi: 10.1186/s12964-024-01979-y

Figure Lengend Snippet: Effect of hCG on tubular structure formation of gubernacular cells. A Representative images of tubular structures analyzed with the dedicated software. Magnification: 4X. B Heat-map representation of the number of junctions and the main of branching length (MBL) of hCG-treated gubernacular cells derived from cryptorchid patients (#94, #2, #7, #20, and #3) and control patients (#116, #40, and #15) at four different time points: after 3 (T1), 6 (T2), 9 (T3), and 12 (T4) hours from the seeding. C Quantitative representation of the number of junctions and MBL at T4, reported as fold change relative to T0 (dashed line). Statistical legend: (*) p < 0.05 for T4 versus T0 for each patient’s derived cells

Article Snippet: B Heat-map representation of the number of junctions and the main of branching length (MBL) of hCG-treated gubernacular cells derived from cryptorchid patients (#94, #2, #7, #20, and #3) and control patients (#116, #40, and #15) at four different time points: after 3 (T1), 6 (T2), 9 (T3), and 12 (T4) hours from the seeding.

Techniques: Software, Derivative Assay, Control

Journal: Cell Communication and Signaling : CCS

Article Title: In vitro effect of hCG on cryptorchid patients’ gubernacular cells: a predictive model for adjuvant personalized therapy

doi: 10.1186/s12964-024-01979-y

Figure Lengend Snippet: hCG treatment impairs oxygen consumption parameters and decreases ROS levels. A Representative immunoblots of phospho- and total-CREB normalized on Amido Black in gubernacular cells derived from patient #20 treated with 100 IU/mL hCG for 10, 20, or 30 minutes compared to untreated (ctrl) cells. The densitometric analysis is the average of three biological replicates. Statistical legend: p < 0.05 (*) for hCG-treated cells versus untreated cells, and p < 0.05 ( # ) for treated cells relative to 20’. B Oxygen Consumption Rate of gubernacular cells derived from pt #20 untreated (control) and treated with 100 IU/mL hCG. Histograms legend: white: untreated (ctrl) cells; grey: hCG-treated (hCG) cells; extrapolated data include basal, maximal respiration, spare respiratory capacity and ATP-driven respiration. C Analysis of intracellular ROS production on gubernacular cells derived from pt #20 untreated (white histogram) and treated with hCG (grey histogram). ROS levels were analyzed every day up to 7 days after hCG treatment. Values are presented as mean ± SEM of three independent biological replicates. Statistical legend: p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***) for hCG-treated cells versus untreated cells. D Representative immunoblots of the mitochondrial protein TOM20 normalized on Amido Black in gubernacular cells derived from patient #20 treated with 100 IU/mL hCG once a week for 4 weeks compared to untreated (ctrl) cells. The densitometric analysis is the average of three biological replicates

Article Snippet: B Heat-map representation of the number of junctions and the main of branching length (MBL) of hCG-treated gubernacular cells derived from cryptorchid patients (#94, #2, #7, #20, and #3) and control patients (#116, #40, and #15) at four different time points: after 3 (T1), 6 (T2), 9 (T3), and 12 (T4) hours from the seeding.

Techniques: Western Blot, Derivative Assay, Control